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anti pan ampar  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti pan ampar
    Anti Pan Ampar, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+pan+ampar/AMPA+Receptor+(GluA2%2F3%2F4)+Antibody/pm41872515-78-48-51
    Average 93 stars, based on 31 article reviews
    anti pan ampar - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: NADPH oxidase-1 suppression prolongs the antidepressant-like effect of ketamine.
    Article Snippet: Protein samples (5-10 μg per lane) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 4–15% gradient gels (Mini-PROTEAN TGX; Bio-Rad) and transferred to polyvinylidene difluoride membranes (Millipore). .. Membranes were blocked with 1% blocking buffer (Perfect-block, MoBiTec) in tris-buffered saline with 0.1% Triton X-100 (TBST) for 1 h at room temperature, followed by overnight incubation at 4 °C with primary antibodies: anti-NOX-1 (1:1000, ab131088, Abcam (for primary neuron samples); 1:1000, GTX103888, GeneTex (for crude brain samples)), anti-pan-AMPAR (1:1000, 2460, Cell Signaling Technology), antiGAD1 (1:1000, 41318, Cell Signaling Technology), anti-Phospho-mTOR (1:1000, 2971, Cell Signaling Technology), anti-Phospho-ERK1/2 (1:1000, 9101, Cell Signaling Technology), anti-NMDA receptor (GluN1, 1:1000, D65B7, Cell Signaling Technology), anti-GAPDH (1:2000, 14C10, Cell Signaling Technology) and anti-β-actin (1:10000, A5441, Sigma). .. Membranes were washed in TBS-T and then incubated with horseradish peroxidase-conjugated secondary antibodies (Peroxidase-conjugated AffiniPure Goat anti-rabbit or anti-mouse, 1:1000, Jackson ImmunoResearch Laboratories) for 1 h at room temperature.

    Saline:

    Article Title: NADPH oxidase-1 suppression prolongs the antidepressant-like effect of ketamine.
    Article Snippet: Protein samples (5-10 μg per lane) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 4–15% gradient gels (Mini-PROTEAN TGX; Bio-Rad) and transferred to polyvinylidene difluoride membranes (Millipore). .. Membranes were blocked with 1% blocking buffer (Perfect-block, MoBiTec) in tris-buffered saline with 0.1% Triton X-100 (TBST) for 1 h at room temperature, followed by overnight incubation at 4 °C with primary antibodies: anti-NOX-1 (1:1000, ab131088, Abcam (for primary neuron samples); 1:1000, GTX103888, GeneTex (for crude brain samples)), anti-pan-AMPAR (1:1000, 2460, Cell Signaling Technology), antiGAD1 (1:1000, 41318, Cell Signaling Technology), anti-Phospho-mTOR (1:1000, 2971, Cell Signaling Technology), anti-Phospho-ERK1/2 (1:1000, 9101, Cell Signaling Technology), anti-NMDA receptor (GluN1, 1:1000, D65B7, Cell Signaling Technology), anti-GAPDH (1:2000, 14C10, Cell Signaling Technology) and anti-β-actin (1:10000, A5441, Sigma). .. Membranes were washed in TBS-T and then incubated with horseradish peroxidase-conjugated secondary antibodies (Peroxidase-conjugated AffiniPure Goat anti-rabbit or anti-mouse, 1:1000, Jackson ImmunoResearch Laboratories) for 1 h at room temperature.

    Incubation:

    Article Title: NADPH oxidase-1 suppression prolongs the antidepressant-like effect of ketamine.
    Article Snippet: Protein samples (5-10 μg per lane) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 4–15% gradient gels (Mini-PROTEAN TGX; Bio-Rad) and transferred to polyvinylidene difluoride membranes (Millipore). .. Membranes were blocked with 1% blocking buffer (Perfect-block, MoBiTec) in tris-buffered saline with 0.1% Triton X-100 (TBST) for 1 h at room temperature, followed by overnight incubation at 4 °C with primary antibodies: anti-NOX-1 (1:1000, ab131088, Abcam (for primary neuron samples); 1:1000, GTX103888, GeneTex (for crude brain samples)), anti-pan-AMPAR (1:1000, 2460, Cell Signaling Technology), antiGAD1 (1:1000, 41318, Cell Signaling Technology), anti-Phospho-mTOR (1:1000, 2971, Cell Signaling Technology), anti-Phospho-ERK1/2 (1:1000, 9101, Cell Signaling Technology), anti-NMDA receptor (GluN1, 1:1000, D65B7, Cell Signaling Technology), anti-GAPDH (1:2000, 14C10, Cell Signaling Technology) and anti-β-actin (1:10000, A5441, Sigma). .. Membranes were washed in TBS-T and then incubated with horseradish peroxidase-conjugated secondary antibodies (Peroxidase-conjugated AffiniPure Goat anti-rabbit or anti-mouse, 1:1000, Jackson ImmunoResearch Laboratories) for 1 h at room temperature.



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    Image Search Results


    Journal: Neuron

    Article Title: Different priming states of synaptic vesicles underlie distinct release probabilities at hippocampal excitatory synapses

    doi: 10.1016/j.neuron.2022.09.035

    Figure Lengend Snippet:

    Article Snippet: Guinea pig polyclonal anti-pan-AMPAR , Nittobo Medical , Cat# panAMPAR-GP-Af580; RRID: AB_2571610.

    Techniques: Recombinant, Electron Microscopy, Plasmid Preparation, Software, Microscopy, Transmission Assay